Ampicillin concentration stock
Moogura/ October 2, 2012/ Eye Care
Ampicillin is one of the synthetic derivatives of penicillin. Transfer all the contents in it. Repeated freeze-thaw cycle can cause ampicillin degradation.
Due to inhibition of cell wall synthesis, bacteria do not grow and have risk to die due to lysis in the presence of ampicillin. However, as the ampicillin concentration decreases below critical level in the surroundings, live bacteria resume the cell wall synthesis and growth.
Ampicillin is one of the most extensively used as selection marker in molecular biology and bacteriology. Mix until all ampicillin is completely dissolved. We recommend to use 15 ml screw-capped tube with millilitre marks. Ampicillin is used to treat many different types of infections caused by bacteria, e. Filter sterilize by passing the solution through 0. It was generated by adding an amino group side chain on the penicillin. Applications A selection marker for bacterial culture during gene cloning experiments.
Follow the table to prepare ampicillin solution of different concentration and volume. Depending on the need, stock solution is diluted to final concentration.
In contrast to penicillin which is active only against gram-positive bacteria, ampicillin is active against both gram-positive and gram negative bacteria. Ampicillin functions as a competitive inhibitor of the enzyme transpeptidase which is required for the synthesis of bacterial cell wall.
Many commonly used vectors have ampicillin resistance gene. It is recommended to store the ampicillin stock solution as small aliquots 1 ml. Ampicillin inhibits the synthesis of cell wall of bacteria by stopping the peptidoglycan cross-linking. Once the content is mixed, make up the volume 10 ml. Aliquoting must be done under sterile condition.
Overview Ampicillin belongs to a group of beta-lactam antibiotics.
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